Commercial-Grade 2kg Master Mix Substrate Bag (All-In-One Grain & 50/50 Mix)

For nearly 20 years, Aussie Mushrooms has set the standard for mycology supplies in Australia. If you are growing high-yielding, wood-loving mushrooms like Oysters, Lion’s Mane, or Chestnuts, this 2kg all-in-one Master Mix bag is the absolute gold standard for achieving massive commercial-sized harvests at home.

Ready to be inoculated with your own Liquid Culture (LC) or spore syringe, this bag is expertly layered to handle both the spawn run and the bulk fruiting phase in one single, highly efficient container.

The “All-In-One” Layered Design

• The Starter Layer (Rye Grain): The bottom of the bag is packed with premium, perfectly hydrated rye grain. This acts as the high-octane starter fuel to get your spore or LC culture running fast and strong.

• The Bulk Layer (Master Mix): The top 2/3 of the bag is filled with the legendary “Master Mix”—a precise 50/50 blend of hardwood sawdust and soy hulls. This high-protein, nutrient-dense formulation is famous in the mycology world for producing absolute monster yields compared to standard sawdust blocks.

The Aussie Mushrooms Difference

Contamination is the enemy of every grower. We process these bags exactly the same way we process the substrate for our own commercial farm.

• True Core Sterilisation: These bags are 100% sterile. They are cooked in our commercial autoclaves, where we actively monitor the core temperatures of the bags—not just the ambient machine temperature—to ensure they hit the exact heat required for the exact amount of time.

• Class 100 Cleanroom Sealing: The bags are cooled and sealed inside our custom, state-of-the-art Class 100 mushroom laboratory. To guarantee our lab environment is flawless, we even leave open, nutrient-filled agar plates around the room while sealing. If the room wasn’t 100% clean, those plates would grow contaminants. They never do.

Crucial Storage & Care Guide

How you store your bags before and during colonisation will dictate your success.

• Stand Them Up: Always stand bags upright so the substrate is away from the white filter patch.

• Keep Filters DRY (Airflow is Mandatory): Filters only work when they are dry! Do not lock your bags in a closed cupboard or sealed box. Without proper airflow, humidity builds up, the filters get wet from the substrate, and contaminants will use that moisture to grow right through the filter.

• Temperature Control: Keep them in a dark, cool area below 20°C (but not in the fridge—fridges are only for putting mushrooms to sleep). Temperatures of 22-24°C or higher will cause the bags to sweat, wetting the filter and pulling in problems. Stable, cool temps prevent sweating. If it is too warm in your state, wait until the cooler seasons to grow.

• Hygiene: Always wear freshly clean clothes when working. Deep clean your work and storage areas monthly to keep the environmental spore load down.

Step-by-Step: Inoculation to Fruiting

1. Prep: Wipe down the built-in self-healing injection port with an isopropyl alcohol swab.

2. Sterilise: Flame-sterilise the needle of your spore or LC syringe until it glows red, then let it cool for a few seconds.

3. Inject: Pierce the port and inject 4 to 10ml of your culture directly into the bottom rye grain layer.

4. Incubate: Store the bag in a clean, dark place with good airflow between 16-20°C.

5. Break & Shake: Wait patiently for the mycelium to fully colonise the bottom rye grain layer (it will turn solid white). Once the grain is fully colonised, massage the bag to break up the grain and thoroughly mix it up into the uncolonised Master Mix layer above.

6. Fruit Like a Kit: Once the mycelium has grown entirely through the Master Mix and consolidated into a solid block, simply cut an ‘X’ or a small slit into the side of the plastic bag, introduce it to fresh air and humidity, and watch it explode with mushrooms!